insertional mutagenesis

insertional mutagenesis
Generally, mutagenesis of DNA by the insertion of one or more bases. Specific examples: (1) oncogenesis by insertion of a retrovirus adjacent to a cellular proto-oncogene;
(2) a strategy of mutagenesis with transposons. After a round of transposition, progeny are screened by PCR, with transposon- and gene-specific primers, for the proximity of the transposon sequence to the gene of interest. As PCR can only produce products up to 1-2 kb, a large fraction of progeny identified as positive by PCR will have a transposon close enough to the gene to inactivate or otherwise alter its pattern of expression.

Dictionary of molecular biology. 2004.

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  • Insertional mutagenesis — is mutagenesis of DNA by the insertion of one or more bases.[1] Insertional mutations can occur naturally, mediated by virus or transposon, or can be artificially created for research purposes in the lab. Contents 1 Signature tagged mutagenesis 2 …   Wikipedia

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  • Mutagenesis — (pronounced /mjuːtəˈdʒɛnɪsɪs/) is a process by which the genetic information of an organism is changed in a stable manner, resulting in a mutation. It may occur spontaneously in nature, or as a result of exposure to mutagens. It can also be… …   Wikipedia

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